Journal: Journal of Nanobiotechnology

Article Title: Hydrogel loaded with cerium-manganese nanoparticles and nerve growth factor enhances spinal cord injury repair by modulating immune microenvironment and promoting neuronal regeneration

doi: 10.1186/s12951-025-03098-3

Figure Lengend Snippet: Optimization of CeMn NP-PEG concentration for biological activity. A Live/dead staining images of PC12 cells treated with H 2 O 2 and various concentrations of CeMn NP-PEG. B Quantification of live cell percentages in PC12 cells treated with H 2 O 2 and different concentrations of CeMn NP-PEG. C Live/dead staining images of BV2 cells treated with H 2 O 2 and various concentrations of CeMn NP-PEG. D Quantification of live cell percentages in BV2 cells treated with H 2 O 2 and different concentrations of CeMn NP-PEG. E ROS detection in PC12 cells using DCFH-DA probe under different treatments. F Quantification of ROS levels in PC12 cells under different treatments. G ROS detection in BV2 cells using DCFH-DA probe under different treatments. H Quantification of ROS levels in BV2 cells under different treatments. Data are presented as mean ± SD. *p < 0.05, **p < 0.01

Article Snippet: PC12 cells (rat adrenal medullary pheochromocytoma differentiated cell line) and BV2 cells (mouse microglia) were purchased from Icell, China.

Techniques: Concentration Assay, Activity Assay, Staining

Journal: Journal of Nanobiotechnology

Article Title: Hydrogel loaded with cerium-manganese nanoparticles and nerve growth factor enhances spinal cord injury repair by modulating immune microenvironment and promoting neuronal regeneration

doi: 10.1186/s12951-025-03098-3

Figure Lengend Snippet: Anti-inflammatory effects of nano-enzyme hydrogel composites in H 2 O 2 -treated PC12 and BV2 Cells. A Relative mRNA expression levels of IL-10, IL-6, IL-4, and IL-1β in PC12 cells treated with PBS, H 2 O 2 , H 2 O 2 + Lightgel, H 2 O 2 + Lightgel/NGF, and H 2 O 2 + Lightgel/NGF/CeMn NP-PEG, as measured by qPCR. B Western blot analysis displaying protein expression levels of IL-10, IL-6, IL-4, IL-1β, and ACTIN in PC12 cells across the different treatment groups. C Quantification of Western blot results for IL-10, IL-6, IL-4, and IL-1β normalized to ACTIN expression in PC12 cells. D Quantification of CCL3 expression levels in PC12 cells. E Relative mRNA expression levels of IL-10, IL-6, IL-4, and IL-1β in BV2 cells treated with the same conditions, assessed by qPCR. F Western blot analysis showing protein expression levels of IL-10, IL-6, IL-4, IL-1β, and ACTIN in BV2 cells. G Quantification of Western blot results for IL-10, IL-6, IL-4, and IL-1β normalized to ACTIN expression in BV2 cells. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: PC12 cells (rat adrenal medullary pheochromocytoma differentiated cell line) and BV2 cells (mouse microglia) were purchased from Icell, China.

Techniques: Expressing, Western Blot

Journal: Journal of Nanobiotechnology

Article Title: Hydrogel loaded with cerium-manganese nanoparticles and nerve growth factor enhances spinal cord injury repair by modulating immune microenvironment and promoting neuronal regeneration

doi: 10.1186/s12951-025-03098-3

Figure Lengend Snippet: ROS-scavenging effects of nano-enzyme hydrogel composites in H 2 O 2 -treated PC12 and BV2 cells. A ROS detection in PC12 cells using DCFH-DA probe under various treatments. B Quantification of relative ROS fluorescence intensity in PC12 cells. C ROS detection in BV2 cells using DCFH-DA probe under various treatments. D Quantification of relative ROS fluorescence intensity in BV2 cells. Data are presented as mean ± SD. *p < 0.05, **p < 0.01

Article Snippet: PC12 cells (rat adrenal medullary pheochromocytoma differentiated cell line) and BV2 cells (mouse microglia) were purchased from Icell, China.

Techniques: Fluorescence

Journal: Journal of Nanobiotechnology

Article Title: Hydrogel loaded with cerium-manganese nanoparticles and nerve growth factor enhances spinal cord injury repair by modulating immune microenvironment and promoting neuronal regeneration

doi: 10.1186/s12951-025-03098-3

Figure Lengend Snippet: Nano-enzyme hydrogel composites promote M2 polarization of H 2 O 2 -treated BV2 cells by inhibiting the cGAS-STING signaling pathway. A Flow cytometry analysis of CD206 expression in BV2 cells treated with PBS, H 2 O 2 , H 2 O 2 + Lightgel, H 2 O 2 + Lightgel/NGF, and H 2 O 2 + Lightgel/NGF/CeMn NP-PEG. B Quantification of the mean fluorescence intensity (MFI) of CD206 in BV2 cells. C Immunofluorescence staining images of CD206 in BV2 cells following various treatments. D Quantification of CD206-positive BV2 cells. E Western blot analysis of cGAS, STING, p-IRF3, IRF3, and ACTIN expression in BV2 cells under different treatments. F Quantification of Western blot results normalized to ACTIN expression. G Immunofluorescence staining images of STING in BV2 cells following different treatments. H Quantification of relative STING fluorescence intensity in BV2 cells. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: PC12 cells (rat adrenal medullary pheochromocytoma differentiated cell line) and BV2 cells (mouse microglia) were purchased from Icell, China.

Techniques: Flow Cytometry, Expressing, Fluorescence, Immunofluorescence, Staining, Western Blot